Established Standard Operating Procedures for S. solfataricus (SOP_SSO_#)
Zaparty et al. 2009, [PubMed]
| Procedure | SOP_SSO_# | SOP | Last update | Contact |
|---|---|---|---|---|
| Strain evaluation and test for genomic stability of S. solfataricus strains P1 and P2 | SOP_SSO_080901 | Test for genomic stability | August 2009 | Christa Schleper |
| Standardized fermentation of S. solfataricus P2 | SOP_SSO_080902 | Minimal medium | August 2009 | John van der Oost |
| SOP_SSO_080903 | Batch fermentation in flasks | August 2009 | John van der Oost | |
| SOP_SSO_080904 | Fermenter set-up and fermentation | August 2009 | John van der Oost | |
| SOP_SSO_080905 | Cell harvest | August 2009 | John van der Oost | |
| SOP_SSO_080906a,b | Preparation S. solfataricus glycerol stocks | August 2009 | John van der Oost | |
| Glucose uptake measurements in S. solfataricus | SOP_SSO_080907a | Preparation of cells | August 2009 | Sonja-V. Albers |
| SOP_SSO_080907b | Glucose uptake measurements | August 2009 | Sonja-V. Albers | |
| Genomics - Reconstruction of the central carbohydrate metabolism (CCM) network by comparative genomics | SOP_SSO_080908 | Reconstruction of the CCM network | August 2009 | Bettina Siebers |
| Comparative genomics | SOP_SSO_080909a | Global identification of putative TFs | August 2009 | Hans-Peter Klenk |
| SOP_SSO_080909b | Identification of putative TFs by psi-BLAST-based approach | August 2009 | Hans-Peter Klenk | |
| SOP_SSO_080909c | Context-based approach for identifying putative TFs of the CCM | August 2009 | Hans-Peter Klenk | |
| Functional Genomics - Transcriptome analyses (Microarray analyses) | SOP_SSO_0809010a | Preparation of mRNA from S. solfataricus cells | August 2009 | John van der Oost |
| SOP_SSO_0809010b | cDNA Synthesis and labeling by reverse transcription | August 2009 | John van der Oost | |
| SOP_SSO_0809010c | Hybridization | August 2009 | John van der Oost | |
| SOP_SSO_0809010d | Scanning, extraction features, normalization and data analyses | August 2009 | John van der Oost | |
| Real-time reverse transcription qPCR | SOP_SSO_060910e | Real-time reverse transcription qPCR | August 2009 | Christa Schleper |
| Proteome analyses | SOP_SSO_0809011a | Cellular extraction | August 2009 | Phillip C. Wright |
| SOP_SSO_0809011b | iTRAQ labeling | August 2009 | Phillip C. Wright | |
| SOP_SSO_0809011c | Strong cation exchange (SCX) | August 2009 | Phillip C. Wright | |
| SOP_SSO_0809011d | Mass spectrometry analysis | August 2009 | Phillip C. Wright | |
| SOP_SSO_0809011e | Data searching | August 2009 | Phillip C. Wright | |
| Metabolome analyses | SOP_SSO_0809012a | Sample preparation | August 2009 | Dietmar Schomburg |
| SOP_SSO_0809012b | GC-MS analysis | August 2009 | Dietmar Schomburg | |
| Biochemistry of the CCM enzymes | SOP_SSO_0809013a | Cloning and heterologous expression in E. coli | August 2009 | Bettina Siebers |
| SOP_SSO_0809013b | Cloning and homologous expression in S. solfataricus | August 2009 | Bettina Siebers | |
| SOP_SSO_0809013c | Preparation of recombinant enzymes | August 2009 | Bettina Siebers | |
| SOP_SSO_0809013d | Preparation of S. solfataricus crude extracts | August 2009 | Bettina Siebers | |
| SOP_SSO_0809013e | Non-phosphorylating glyceraldehyde-3-phosphate (GAP) dehydrogenase (GAPN; E.C. 1.2.1.9) and gluconate dehydratase (GAD; EC 4.2.1.39) activity in cell-free extracts | August 2009 | Bettina Siebers | |
| SOP_SSO_0809013f | Western blotting and detection of the recombinant S. solfataricus proteins | August 2009 | Bettina Siebers |